Mud crab

I. Problem/QuestionThere is shortly an increasing commercial viability of mud crabs especially in semitropical countries. However, due to seasonal factors affecting the spawning of these species, there a need to break up methods for year-around larval production (Zeng, 2007, p. 1478-1479) arises. There are no latest techniques that would enable this process. Therefore, this study investigated on the feasibility of out-of-season mud crab spawning induction. It besides desexualised whether in vitro incubation of eggs is possible. An evaluation of the temperature effects on the rates of embryonic development of mud crabs was also conducted. This is in order to provide predicted dates of hatching for berried mud crab females (Zeng, 2007, p. 1478-1479). II. observational procedures/MethodThe researchers kept female individuals of S. paramamosain in 1000L tanks. These holding tanks contained seawater, filtered with sand, with constant salinity of 29-32 g/L but with uncontrolled tempe rature between 10-30 C. In order to determine the proximate maturation index (PMI) (Zeng, 2007, p. 1479), crab ovarian development was regularly checked every fortnight using a calliper to judge the width of the strip of lightly in the carapace when shone with bright light from underneath (Zeng, 2007, p. 1479).The induction experiment was started with the random selection of mature subjects with